Fig. 1: The reciprocal polarity of Bnl presentation and reception via cytonemes. | Nature Communications

Fig. 1: The reciprocal polarity of Bnl presentation and reception via cytonemes.

From: GPI-anchored FGF directs cytoneme-mediated bidirectional contacts to regulate its tissue-specific dispersion

Fig. 1

a Drawing depicting the organization of the ASP, wing disc, myoblasts, and Btl-containing ASP cytonemes receiving Bnl from the disc bnl source. b Spatial organization of the wing disc bnl-source (bnl-LexA,LexO-mCherryCAAX) and ASP (btlGal4,UAS-CD8:GFP) cytonemes (arrow). c–e’ Polarized Bnl presentation from the source orienting toward the ASP; c polarized clustering of externalized Bnlex (red; αBnlex) at the contact sites of the unmarked source (dashed lined area) and Btl:GFP-containing ASP cytonemes (Btl:GFP - btl:GFP fTRG, see Supplementary Table 4); d, d’, e, e’ polarized cytoneme-mediated presentation of overexpressed Bnl:GFP (d-d”; UAS-mCherryCAAX/+; bnl-Gal4/ UAS-Bnl:GFP) and endogenous Bnl:GFPendo (e, e’; UAS-mCherryCAAX/+; bnl-Gal4/bnl:gfpendo) from the mCherryCAAX-marked bnl-source, orienting toward the overlaying ASP (dashed line); dashed arrow, Bnl:GFP puncta in internalized vesicles9 within the ASP (dashed line); arrow, Bnl:GFP puncta on source cytonemes; e, e’ airyscan image; d” Bnl:GFP intensity plot within the boxed source area in d’ along the proximal (p)-to-distal (d) direction (arrows) relative to the ASP, showing selective enrichment of the overexpressed signal toward the ASP. f–h” 3D-rendered images showing the ASP-specific polarity of source cytonemes (arrows); f, g mCherryCAAX-marked source and nlsGFP-marked ASP (btl-Gal4,UAS-nlsGFP/+; bnl-LexA,LexO-mCherryCAAX/+); h, h’ CD8:GFP-expressing mosaic clones within the bnl source area (see Methods); h” violin plot displaying the source cytoneme length distribution (see Supplementary Fig. 1d). i–n Contact-dependent reciprocal guidance of source (red) and ASP (green) cytonemes (btl-Gal4,UAS-CD8:GFP/+; bnl-LexA,LexO-mCherryCAAX/+); arrowhead, contact site; j illustration of i–k”; l–n violin plots displaying ASP (green) and source (red) cytoneme dynamics as indicated (also see Supplementary Fig. 1e–h and Supplementary Table 1 for statistics). All except c–e’, live imaging. Violin plots: black dotted lines - median and 25th and 75th percentiles. Source data are provided as a Source data file. Scale bars, 20 μm; 5 μm (e, e’, h, h’).

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