Fig. 5: Bnl is GPI-anchored to the wing disc source cell surface. | Nature Communications

Fig. 5: Bnl is GPI-anchored to the wing disc source cell surface.

From: GPI-anchored FGF directs cytoneme-mediated bidirectional contacts to regulate its tissue-specific dispersion

Fig. 5

a–c Wing discs (w− showing surface-localized native Bnlex (red, αBnlex) levels on the bnl-source area (arrows) before (a) and after (b) PI-PLC treatment; c box plots comparing numerical values as indicated, p = 0.0013 (**; unpaired two-tailed t test). d–f Wing discs overexpressing Bnl under bnl-Gal4 showing surface Bnlex (red, αBnlex) levels on the bnl-source (arrows) before (d) and after (e) PI-PLC treatment; f box plots comparing numerical values as indicated, p = 0.024 × 10−4 (***; unpaired two-tailed t test). g–m Source surface levels (red, αGFPex) of Bnl:GFP, Bnl:GFPΔC and Bnl:GFPΔC-TM on wing discs when expressed under bnl-Gal4 before and after PI-PLC; asterisks, non-expressing source-surrounding disc area; dashed line, ASP or bnl-source; m, box plots comparing the fraction of surface localized (red, αGFPex) to total protein (probed by GFP) of Bnl:GFP variants on the source before and after PI-PLC treatment; n = 5 biologically independent samples for each; p values—p < 0.05 (*) and p < 0.01 (**) (one-way ANOVA followed by Tukey HSD test). All box plots: box shows the median, 1st quartile, 3rd quartile, and whiskers show minimum and maximum. Source data are provided as a Source data file. Scale bars, 30 μm.

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