Fig. 6: GPI-anchored Bnl acts as a CAM. | Nature Communications

Fig. 6: GPI-anchored Bnl acts as a CAM.

From: GPI-anchored FGF directs cytoneme-mediated bidirectional contacts to regulate its tissue-specific dispersion

Fig. 6

a–c CAM-like polarized trans-pairing of S2-Btl:Cherry with either S2-Bnl:GFPΔC-TM (b, c) or S2-Bnl:GFP (c; see Fig. 3g, k) but not with S2-Bnl:GFPΔC (a, c); arrow, polarized receptor-ligand co-clusters at the synaptic site; open arrow, nucleus in source proximal S2-Btl:Cherry (a) and trans-paired S2-Btl:Cherry (b); arrowhead, Bnl:GFP signal uptake into the juxtaposed or trans-paired S2-Btl:Cherry cell; blue, nuclear dpERK (αdpERK); c bar graphs comparing the mean (±SD) frequency of receptor-ligand trans-pairing for GPI-modified and non-GPI modified Bnl:GFP variants from three independent experiments (see Methods); p values were obtained by one-way ANOVA followed by Tukey HSD test; total GFP-positive + Cherry-positive cells analyzed: 1916 (Bnl:GFP + Btl:Cherry), 2664 (Bnl:GFPΔC + Btl:Cherry), 2192 (Bnl:GFPΔC-TM + Btl:Cherry). d–l Comparison of Bnl:GFP (control), Bnl:GFPΔC, or Bnl:GFPΔC-TM signals for induction of reciprocal polarity of ASP and source cytonemes (arrows), when expressed from the disc source; genotypes, as indicated; d inset, ROI (dashed box) in green and blue channels; d, g, j extended Z projection; e, e’, h, k 3D-rendered views; dashed lines, ASP; g, h dashed arrows, randomly oriented short cytonemes; f, i, l R-plots comparing numbers, length, and directionality of ASP and source cytonemes as indicated; n = number of discs analyzed (also see Supplementary Fig. 6a–g). Source data are provided as a Source data file. All panels except a, b, live imaging. Scale bars, 10 μm (a, b), 20 μm (d–k).

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