Fig. 9: GPI-anchored Bnl promotes recipient-specific patterning. | Nature Communications

Fig. 9: GPI-anchored Bnl promotes recipient-specific patterning.

From: GPI-anchored FGF directs cytoneme-mediated bidirectional contacts to regulate its tissue-specific dispersion

Fig. 9: GPI-anchored Bnl promotes recipient-specific patterning.The alternative text for this image may have been generated using AI.

a–c Comparison of non-autonomous signaling (dpERK, red; arrows) patterns of Bnl:GFP (control), Bnl:GFPΔC, and Bnl:GFPΔC-TM, expressed from ectopic GOF clones within the ASP stalk and TC; approximate clone locations and dpERK patterns indicated in inset; arrow and arrowhead, signal-recipient cells with and without nuclear dpERK, respectively; genotype: hsFlp; btlenh > y+>Gal4,btlenh-mRFPmoe x UAS-“X”. d Violin plots comparing the percentage of signal-receiving ASP cells with nuclear dpERK; clone, clonal expression; OE, overexpression (bnlGa4 x UAS-X; see Supplementary Fig. 7h–k); for OE: p < 0.01 for ΔC (n = 16) vs either Bnl:GFP (n = 17) or TM (n = 13) (one-way ANOVA followed by Tukey HSD test); for clonal analyses: see Supplementary Fig. 7g for statistics. e–h Levels of tracheal branch invasion (arrows) into larval salivary glands ectopically expressing either Bnl:GFP (control), TM, or ΔC under bnl-Gal4 (bnl-Gal4 X UAS-X); e–g brightfield images, ×10 magnification. h Graphs showing mean frequency (±SD) of terminal branching (see Methods); n, number of tissues analyzed per genotype: 5 (TM), 6 (Bnl:GFP), 4 (ΔC). i–l Levels of Bnl:GFP, TM, and ΔC displayed on the basal surface of the expressing salivary glands; arrow, cell junctions. l Violin plots comparing the fraction of surface-displayed signals (red/arrowhead, αGFPex) to total protein expressed (GFP level); n, number of salivary glands; p < 0.05: Bnl:GFP vs. TM, p < 0.01: ΔC vs. either Bnl:GFP or TM (one-way ANOVA followed by Tukey HSD test). Violin plots, black dotted lines show the median as well as 25th and 75th percentiles. Source data are provided as a Source data file. Scale bars, 30 μm; 100 μm (e–g); 20 μm (i–k′).

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