Fig. 4: A change in progenitor cell dynamics and morphology is observed upon Dlgap4 KD.

a, b Representative images and quantifications of Pax6⁺ (red) and Blbp-GFP⁺ (white) cells in E15.5 brains (n = 7 embryos from 3 litters per condition). Means and relativized individual values ± SEM are shown. *p = 0.027 by two-sided unpaired t test. c Pax6⁺-Blbp-GFP⁺ cell distribution along the cortical wall divided into 10 bins. Two-way ANOVA: Interaction Bin × ShmiRNA condition: F_9,160 = 2.76, p = 0.0049; **p < 0.008 by Sidak’s multiple comparison test. d, e Representative images and quantifications of Tbr2⁺ (red) and Blbp-GFP⁺ (white) cells in E15.5 brains (n = 6 embryos from 3 litters per condition). Means and relativized individual values ± SEM are shown. Two-sided unpaired t-test, * p = 0.041. f Quantification of Tbr2⁺Blbp-GFP cells along the cortical wall. Analysis was performed by two-way ANOVA followed by Sidak’s multiple comparisons test. Interaction Bin x ShmiRNA, F_{9, 270} = 5.13, p < 0.0001; * p < 0.04, *** p = 0.0003. g Analysis of cellular shape and morphology of Tbr2⁺Blbp-GFP⁺ cells in ShmiRNACtl and ShmiRNADlgap4 KD conditions. Arrows indicate examples of double-labeled Tbr2⁺Blbp-GFP⁺ cells in IUE experiments. h The number of primary processes, i process length, and j soma surface, were analyzed in Tbr2⁺Blbp-GFP⁺ cells in both ShmiRNACtl and ShmiRNADlgap4 KD conditions (n = 57 ShmiRNACtl and n = 68 ShmiRNADlgap4 KD cells from n = 5 embryos per condition). Means and individual values ± SEM are shown. *p < 0.05 by two-sided unpaired t-test. Images correspond to 10 μm stack projections.