Fig. 8: Dlgap4 KD or OE leads to slowed neuronal migration.

a Left: Schematic representation of the electroporated ROI at P0. Right: Representative Cux1 (red) labeling and Blbp-GFP (white) in Ctl and Dlgap4 KD brains at P0. b Cux1 thickness relativized to cortical wall thickness (n = 3 Ctl, n = 4 Dlgap4 KD brains from 2 litters per condition). Quantification data represent relativized individual values, mean ± SEM (two-sided unpaired t-test, *p = 0.018). c Total Cux1⁺ cells per ROI (n = 5 Ctl, n = 8 Dlgap4 KD brains from 2 litters per condition). ROI: 135 μm height × 370 μm width. Quantification data represent individual values, mean ± SEM (two-sided unpaired t-test, **p = 0.0066). d GFP⁺ cells are still migrating below the CP in the KD at P0 (e.g., red arrows). e Schematic representation of the electroporated ROI at E18.5. A Dcx-GFP reporter promoter vector was co-electroporated with a pDcx-DLGAP4 WT-Flag-ires-GFP or pDcx-DLGAP4 MUT-Flag-ires-GFP construct. f Quantification of Dcx-GFP⁺ cells along the cortical wall divided in 10 bins. Two-way ANOVA followed by Sidak’s test was performed (n = 3 brains from 2 litters per condition). Relativized mean values ± SEM are shown. Two-way ANOVA, Interaction Bin x OE condition, F_{9, 60} = 16.11, p < 0.0001; *p = 0.014, **p < 0.005, ***p = 0.0004, ****p < 0.0001. g Representative images of OE experiments targeting specific neuronal migration after IUE showing Dcx-GFP⁺ (green) cell distribution and Hoechst staining. h Total Cux1⁺ Dcx-GFP⁺/ Dcx-GFP⁺ cells did not change along conditions. Relativized mean and individual values ± SEM are shown. One-way ANOVA followed by Tukey’s test, F_{2, 6} = 0.39, p = 0.70. i Representative images of OE experiments showing Cux1⁺ (magenta) and Dcx-GFP⁺ (green) cell distribution. j Quantification of Cux1⁺ Dcx-GFP⁺/Dcx-GFP⁺ cells along the cortical wall divided in 10 bins. Two-way ANOVA followed by Sidak’s test was performed (n = 3 from 2 litters per condition). Relativized mean values ± SEM are shown. Two-way ANOVA, Interaction Bin × OE condition, F_{9, 60} = 16.63, p < 0.0001; *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001.