Fig. 6: Phase separation of SS18-SSX1 and binding to BRG1 are important for the oncogenic activity of SS18-SSX1. | Nature Communications

Fig. 6: Phase separation of SS18-SSX1 and binding to BRG1 are important for the oncogenic activity of SS18-SSX1.

From: Phase transition and remodeling complex assembly are important for SS18-SSX oncogenic activity in synovial sarcomas

Fig. 6: Phase separation of SS18-SSX1 and binding to BRG1 are important for the oncogenic activity of SS18-SSX1.

a The protein level of SS18-SSX1 WT or mutants overexpressed in NIH3T3 cells. b EdU assay of NIH3T3 cell lines overexpressed SS18-SSX1 WT or mutants. EdU positive nuclei (Alexa Fluor 488 azide labeled; green) and 1× Hoechst stained nuclei of all the cells (blue) were visualized by fluorescence microscopy. c Statistical graph of the EdU assay in (b). Error bars represent mean ± SEM, one-way ANOVA, Tukey’s multiple comparisons test; n = 5 independent experiments, ****P < 0.0001. d Plate colony formation of NIH3T3 cell lines overexpressing SS18-SSX1 WT and mutants. Colonies were stained with 0.1% crystal violet. Cell migration (e) and invasion g assay of NIH3T3 cell lines overexpressing SS18-SSX1 WT and mutants. Cells were stained with 1% crystal violet and visualized by bright-field microscopy. f, h Statistical graph of the cell migration and invasion assay. Error bars represent mean ± SEM, one-way ANOVA, Tukey’s multiple comparisons test; n = 3 independent experiments, ****P < 0.0001.

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