Fig. 3: Adult zebrafish cardiomyocytes activate nkx2.5 expression following injury.
From: Activation of Nkx2.5 transcriptional program is required for adult myocardial repair
A–C Representative images of RNAscope in situ hybridization on sections of uninjured (n = 3) (A) and injured wild-type hearts at 7 dpa (n = 3) (B), and 14 dpa (n = 3) (C) highlight nkx2.5 expression in the entire ventricle, expanding into the regenerate. Dashed lines represent amputation planes. Scale bar, 50 μm. D–F′ Co-localization of Tg(nkx2.5:ZsYellow) and Tropomyosin delineates nkx2.5 expression in the myocardium in both uninjured (n = 4) (D) and injured wild-type hearts at 7 dpa (n = 5) (E) and 14 dpa (n = 6) (F). Higher magnification panels (D′–F′) indicate the areas outlined in the rectangular boxes. Scale bar, 100 μm. G-H‴) Anti-Raldh2 (n = 4) (G–G‴) and anti-Vimentin (n = 5) (H–H‴) immunostainings in Tg(nkx2.5:ZsYellow) fish highlight the myocardial-specific expression of nkx2.5. Higher magnification panels (G′–G‴, H′–H‴) indicate the areas outlined in the rectangular boxes. Scale bar, 50 μm.
