Fig. 8: Nkx2.5 is required for cardiomyocyte proliferation and dedifferentiation in the regenerating heart.
From: Activation of Nkx2.5 transcriptional program is required for adult myocardial repair
A–D Representative images of PCNA/Mef2 immunostaining in wild-type (n = 8 in A, n = 6 in C) and nkx2.5−/− (n = 8 in B, n = 6 in D) hearts indicate proliferating CMs (arrows). Scale bar, 100 μm. E Quantification of PCNA+Mef2+ cells in wild-type and nkx2.5−/− hearts. n = 8 at 7 dpa and n = 6 at 14 dpa for both genotypes. Mean and standard error of each data set is shown. Unpaired, two-tailed t-tests show statistical differences between WT7 and MT7 (p = 0.0033), WT7 and WT14 (p = 0.0143), and MT7 and MT14 (p = 0.0369). F, G Representative images of embCMHC/MF20 immunostaining illustrating diminished expression of immature myosin in the nkx2.5−/− (n = 6) compared to the wild-type (n = 6) wound border. Scale bar, 100 μm. H Quantification of integrated signal density of the embCMHC stain is depicted in wild-type (n = 6) and nkx2.5−/− (n = 6) injury borders. Mean and standard error of each data set are shown. Unpaired, two-tailed t-test indicates a statistically significant difference (p = 0.0248). Source data are provided as a Source Data file.
