Fig. 4: Efficiency of mutagenesis affects DNA repair outcomes.
From: Cas9-induced large deletions and small indels are controlled in a convergent fashion
A Relationship between flow cytometry samples. gRNA #48U is a weaker version of #48. N = 4–6 biologically independent replicates. B Relationship between clones based on their indel profiles, analogous to Fig. 2B. Only non-significant clones are shown for clarity. Biological replicates (N = 2) were averaged. C Difference in indel frequency between the regular gRNA #48 and its less active counterpart #48U. Same display conventions as in Fig. 2. D Relationship between clones based on their indel profiles, analogous to Fig. 2B. Each clone is colored by the frequency of mutagenesis assayed by flow cytometry on day 14 using gRNA #48U, a proxy for Cas9 activity. For clarity, only non-significant clones are depicted.
