Fig. 3: Yki signaling is required for the proliferation and migration of tracheal progenitors.
From: Metabolic control of progenitor cell propagation during Drosophila tracheal remodeling
a–c The incorporation of EdU in the tracheal progenitors labeled by P[B123]-RFP-moe of pupae of indicated ages APF. d Scatter plot showing the number of EdU incorporation in Tr4 and Tr5 progenitors. n = 9, 9, 8, 7, 9, 8 from the left to the right. e–i Expression of ykiRNAi decreased the proliferation of progenitor, but did not cause apoptosis. Staining tracheal progenitors of white pupae with antibodies against EdU (e, f) or cleaved Caspase3 (h, i). g Bar plot depicts the incorporated EdU in the Tr4 tracheal progenitors of control (n = 9) and ykiRNAi flies (n = 6). *****p = 3.44e-6. h, i Three independent experiments were repeated with similar results. j–k” Dependence of progenitor migration on Yki. Arrowheads point to tracheal progenitors. l Scatter plot showing the velocity of migrating progenitors. n = 8 for each group. Error bars represent standard deviation. p value: Tr4 (p = 7.42e-8) and Tr5 (p = 2.86e-12). d, g, l Three biologically independent experiments were performed. Results are presented as mean values ± SD. No adjustments were made for multiple comparisons. Unpaired two-tailed t-test was used for all statistical analyses in this figure. Scale bars: 30 μm (a–c, e, f, h, i) and 300 μm (j–k”). Genotypes: (a–c) P[B123]-RFP-moe/+; (e, h, j–j”) btl-Gal4/+; P[B123]-RFP-moe/tub-Gal80ts; (f, i, k–k”) btl-Gal4/ UAS-ykiRNAi; P[B123]-RFP-moe/tub-Gal80ts. Source data for (d, g, l) are provided as a Source Data file.
