Fig. 4: Insulin signaling inhibits the activity of Yki signaling.
From: Metabolic control of progenitor cell propagation during Drosophila tracheal remodeling
a–c Differential expression profiling in tracheal progenitors of InRDN pupae. a Volcano plot of RNA-seq showing differentially regulated genes up- and downregulated genes with four-fold and higher changes (upregulated genes in red; downregulated genes in blue) in InRDN compared with control. Complete differentially expressed genes (DEGs) from RNA-seq results were listed in Supplementary Fig. 1d. b GO analysis reveals top functional clusters among the differentially expressed genes. Hippo signaling pathway denoted in dashed box is in the list with high enrichment score. c Heatmap showing the differential expression of genes from two top enriched GO groups: Hippo pathway and FOXO pathway in InRDN relative to control. d–e’ Expression of InRDN promoted the activity of Yki. Staining tracheal progenitors of btl > GFP in control (d, d’) and in the presence of InRDN (e, e’) with α-lacZ antibodies (magenta). Three independent experiments were repeated with similar results. d’, e’, Merge images. f–i Elevation of insulin perturbs Yki signaling. f–g’ The expression of ex-lacZ was decreased upon the administration of insulin. Merge images (f’, g). h, i The confocal images showing staining tracheal progenitors of (btl > YAP-SPARK) in control and insulin-treated pupae with α-lacZ antibodies. Merge images showing YAP-SPARK in green and ex-lacZ in magenta. The progenitors are outlined by dashed lines. Arrowheads denote the GFP droplets of YAP-SPARK sensors in tracheal progenitors. j Bar graph plots the expression of ex-lacZ normalized to GFP signal from btl > GFP. n = 7 for each group. ***p = 3.63e-4. k Bar graph represents SPARK signal in control (n = 8) and insulin-treated pupae (n = 13). *****p = 3.13e-6. j, k Three biologically independent experiments were performed. Results are presented as mean values ± SD. Unpaired two-tailed t-test was used for all statistical analyses. Scale bars: 50 μm (d–i). Genotypes: (d, d’) ex-lacZ/+; btl-Gal4,UAS-GFP/ tub-Gal80ts; (e, e’) ex-lacZ/UAS-InRDN; btl-Gal4,UAS-GFP/tub-Gal80ts; (f, f’, g, g’) ex-lacZ/+; btl-Gal4,UAS-GFP/ + ; (h, i) btl-Gal4/ex-lacZ; UAS-YAP-SPARK+. No adjustments were made for multiple comparisons in this figure. Source data for (j, k) are provided as a Source Data file.
