Fig. 5: The effects of insulin on progenitor migration.
From: Metabolic control of progenitor cell propagation during Drosophila tracheal remodeling
a–d An ex vivo culture condition with a key supplement of steroid hormone 20-hydroxyecdysone (20E) was sufficient to preserve the migration of tracheal progenitors. e, f Exogenous insulin prevents the migration of tracheal progenitors. Pupal trachea in Grace’s Insect Medium (GIM) (a, b), ecdysone (20E) plus GIM (c, d) and ecdysone (20E) plus GIM with insulin (e, f). The positions of progenitors at 0 h APF and 1 h APF are indicated by dashed lines or solid lines, respectively. g Scatter plot depicting migration distance of tracheal progenitors. Five biologically independent experiments were performed. Tr4: plain (n = 10), 20E (n = 8; p = 4.47e-8), 20E&insulin (n = 6; p = 0.253). Tr5: plain (n = 9), 20E (n = 8; p = 5.48e-4), 20E&insulin (n = 6; p = 0.669). Data are presented as mean values ± SD. N.S. not significant. Unpaired two-tailed t-test was used for all statistical analyses. No adjustments were made for multiple comparisons. h, i Reduced insulin activity promotes migration of tracheal progenitors. Lowering insulin activity by expressing InRDN for 20 h in L3 triggered early migration of tracheal progenitors in white pupae. Five independent experiments were repeated with similar results. Arrow denotes the progression of progenitors on dorsal trunk. Scale bars: 200 μm (a–f, h, i). Genotypes: (a–f) P[B123]-RFP-moe/+; (h) btl-Gal4/+; tub-Gal80ts/ P[B123]-RFP-moe; (i) btl-Gal4/UAS-InRDN; tub-Gal80ts/ P[B123]-RFP-moe. Source data for (g) are provided as a Source Data file.
