Fig. 7: Identification of gene targets of Yki in Drosophila tracheal progenitors.
From: Metabolic control of progenitor cell propagation during Drosophila tracheal remodeling
a–c Localization of Yki on chromosomes in trachea. a ChIP-seq peaks at loci regulated by Yki. Scale bar: 5 kb, except for Ncad (10 kb). Enrichment was normalized input. b Bubble plot represents GO analysis showing top functional clusters among gene targets. c Cis-regulatory elements and corresponding transcription factors in the gene targets of Yki. b, c No adjustments were made for multiple comparisons. d Heatmap depicting differentially expressed genes (DEGs) in tracheal progenitors between control and ykiRNAi flies. e Venn plot showing comparison between DEGs from RNA-seq and gene targets of ChIP-seq with Yki antibody. f–m’ Validation of gene targets of Yki ChIP-seq. Two independent experiments were repeated with similar results. f, g Staining tracheal progenitors with α-Hnt antibody. h, i GFP fluorescence of Dally:YFP in the tracheal progenitors of control and ykiRNAi flies. j–m’ Staining tracheal progenitors of control (j, j’, l, l’) and ykiRNAi-expressing flies (k, k’, m, m’) with α-Dlp and α-Ncad antibodies. j’, k’, l’, m’, Merge images. Genotypes: (f, j, j’, l, l’) btl-Gal4/+; P[B123]-RFP-moe/tub-Gal80ts; (g, k, k’, m, m’) btl-Gal4/UAS-ykiRNAi; P[B123]-RFP-moe/tub-Gal80ts; (h) btl-Gal4/+; dally:YFP/tub-Gal80ts; (i) btl-Gal4/ UAS-ykiRNAi; dally:YFP/tub-Gal80ts. Scale bars: 50 μm (f–m’).
