Fig. 5: Material properties of enzymatically active full-length Dhh1, enzymatically deactivated Dhh1^DQAD, and the LCD-lacking variant Dhh1^48–425.

The reciprocal of the relative diffusion coefficient (1 / Relative Diffusivity = 〈D₀〉 / D(t)) of the condensates as well as the fraction of dynamically arrested gel-/glass-like droplets were measured over a time course of 5 days. D₀ and D(t) are diffusion coefficients at time zero and time t, respectively. Each dot represents a single droplet. a Full-length Dhh1 droplets remain liquid over several days (black dots) and only a fraction of the droplets becomes dynamically arrested on day 5 (red dots). These droplets deviate from liquid-like behavior and are therefore displayed outside the graph. b Representative ISFs of droplets of full-length Dhh1 on day 0 (high-diffusive, green) and day 5 (high-diffusive, green; arrested, gray). c Droplets formed with full-length Dhh1 could be reversibly dissolved upon dilution on day 0 but not anymore on day 5, confirming the presence of non-liquid, or low-diffusive structures. d In addition to high-diffusive droplets, the catalytically deactivated Dhh1^DQAD variant showed a small fraction of dynamically arrested droplets already on day 0. The number of dynamically arrested droplets increased on day 2 and was accompanied by high- and low-diffusive droplets. e Typical ISFs of Dhh1^DQAD droplets, showing high-diffusive (green) and arrested droplets (gray) on day 0 and high-, low-diffusive (blue) and arrested droplets on day 5. f Also the Dhh1^DQAD droplets could not be dissolved by dilution on day 5, in contrast to full dissolution on day 0. g Droplets formed in the presence of Dhh1^48–425 showed a decrease of the diffusivity of about one order of magnitude with respect to Dhh1 after day 1. No dynamically arrested droplets were observed over the time course of 5 days. h Representative ISFs of Dhh1^48–425 droplets with high diffusivity on day 0 (green) and of droplets with low diffusivity observed after day 1 (blue). i Also Dhh1^48–425 droplets could be dissolved on day 0 but not on day 5. Source data for panels a, d, g are provided in the Source Data file.