Fig. 3: A distal enhancer regulates CPA activity of proximal PAS.
From: Enhancers regulate 3′ end processing activity to control expression of alternative 3′UTR isoforms
a UCSC genome browser snapshot showing the genomic context of the NUDT21 gene locus. The region with the local maximum of acetylated H3K27 measured by ChIP-seq in MCF7 cells was defined as distal enhancer (Denh). b Schematic of reporter constructs used to investigate enhancer-dependent CPA activity in the context of three promoters. The GAPDH promoter (Gprom) drives a single-UTR gene, whereas the Pprom and NUDT21 (Nprom) promoters drive multi-UTR genes. Shown as in Fig. 2a. c Enhancer-dependent transcriptional activity of the Gprom shown as in Fig. 2b. Data are shown as mean ± std of n = 3 biologically independent experiments. Two-tailed t-test for independent samples was performed. **P = 0.0005. d Enhancer-dependent CPA activity in the context of the Gprom shown as in Fig. 2c. Data are shown as mean ± std of n = 3 biologically independent experiments. Two-tailed t-test for independent samples was performed. NS, not significant. e As in (c), but enhancer-dependent transcriptional activity of two multi-UTR gene promoters is shown. Data are shown as mean ± std of n = 3 biologically independent experiments. Two-tailed t-test for independent samples was performed. NS not significant. f As in (d), but enhancer-dependent CPA activity in the context of the Pprom is shown. Data are shown as mean ± std of n = 3 biologically independent experiments. Two-tailed t-test for independent samples was performed. **P = 1 × 10−8. g As in (d), but enhancer-dependent CPA activity in the context of the Nprom is shown. Data are shown as mean ± std of n = 3 biologically independent experiments. Two-tailed t-test for independent samples was performed. **P = 1 × 10−5. Source data for figures (c–g) are provided as a Source Data file.
