Fig. 4: Transcription and transcription elongation factors are widespread regulators of CPA activity.

a As in Fig. 1a but shown are the binding sites of transcription factors that were knocked-down individually. b Schematic of reporter constructs to identify transcription factors (TFs) and co-activators that regulate CPA activity in the context of the Penh-Pprom reporter. Ctrl KD, control knock-down. c Summary of PTEN PPAS CPA activity and fold repression of Tx activity obtained in the shRNA screen. Shown is the mean in ctrl KD and transcription factor KD samples for the Penh-Pprom reporter. The values are reported in Table 1 and the replicates are shown in Supplementary Fig. 4a, b. The shaded areas denote the extent of change in CPA activity in TF KD samples (white, change is not significant; gray, significant change; dark gray, more than 0.30 change in CPA activity). d CPA activity of additional PAS when transcribed from the Penh-Pprom reporter. Shown is mean ± std of n = 3 biologically independent experiments after transcription factor KD or mutation of MYC-binding sites (MYC-binding site mut) in the Penh. Two-tailed t-test for independent samples was performed; *P < 0.03; NS not significant. Source data are provided as a Source Data file. e UCSC genome browser snapshot showing the PTEN gene locus with ChIP-seq data for MYC and the sequence conservation track of 100 vertebrates. The position of the two conserved MYC-binding sites (canonical E-boxes) are indicated.