Fig. 4: Control functional experiments.
From: Structural mechanism of TRPV3 channel inhibition by the anesthetic dyclonine
a Dose–response curves for the F666A mutant and wild-type TRPV3 activation by 2-APB. The changes in F340/F380 were normalized to their approximated maximal values at saturating concentrations of 2-APB. Curves through the points are fits with the logistic equation and EC50 = 27.4 ± 4.5 µM and nHill = 1.19 ± 0.09 (n = 4 independent experiments) for wild-type TRPV3 and EC50 = 20.7 ± 0.9 µM and nHill = 0.80 ± 0.02 (n = 3 independent experiments) for F666A. The data for wild-type TRPV3 have been published before1. b Dose–response curves for inhibition of the F666A mutant and wild-type TRPV3 by osthole. The changes in F340/F380 were normalized to their maximal values in the absence of osthole. Curves through the points are fits with the logistic equation and IC50 = 20.5 ± 0.5 µM and nHill = 1.84 ± 0.14 (n = 4 independent experiments) for wild-type TRPV3 and IC50 = 33.1 ± 3.8 µM and nHill = 1.37 ± 0.19 (n = 3 independent experiments) for F666A. The data for wild-type TRPV3 have been published before2. c Double logarithmic Schild plot for 2-APB concentration dependencies of TRPV3 activation in the presence of 10 μM (pink circles, n = 3 independent experiments) and 60 μM (green circles, n = 3 independent experiments) of dyclonine. Lines through the points of the corresponding color are linear fits. For all panels, data points are presented as mean ± SEM and source data are provided.
