Fig. 4: LPS stimulation of distinct subtypes of macrophages.
From: Robust temporal map of human in vitro myelopoiesis using single-cell genomics
a Schematic illustration of the in vitro differentiation protocol and cell-type annotation analysis, steps before the macrophage differentiation phase are hidden. Additional experimental conditions (LPS stimulation at distinct time points) are highlighted in red. b UMAP projections of scRNAseq analysis of LPS stimulated samples and matched controls for 4 populations of macrophages. c (top) Dot plot of genes overexpressed >3 log fold-change in either of the 4 LPS stimulated samples vs their control, (bottom) Dot plot of genes significantly overexpressed in 1 of the 4 populations analysed. d Cytokines protein expression levels in supernatants after LPS stimulation (n = 2) and controls (n = 1) for the 4 experimental conditions analysed. Media was collected from samples processed for scRNAseq (shown in b, c). Source data are provided in the Source data file.
