Fig. 4: Assessment of HBV infection in RM livers.
From: Long-term hepatitis B virus infection of rhesus macaques requires suppression of host immunity
A hNTCP expression in the liver is consistent over time following HBV infection. B HBV DNA levels are consistent over time following HBV infection. C HBV expression is consistent over time following HBV infection. D Distribution of HBV DNA in the liver lobes at euthanasia. E Distribution of HBV RNA in the liver lobes at TOD. F Immunofluorescence shows wide-spread HBc (green) and HBs (red) infection of hepatocytes, but not Kupffer cells (yellow). G Dual fluorescent RNAScope-IHC staining shows HBV RNA (red) and HBcAg expression (green) in the liver of infected RM. H Frequency of HBcAg-positive nuclei in the liver of HBV-infected RM. I Quantification of CD3 + T cells in the liver of HBV-infected RM. J cccDNA levels in RM liver following HBV infection. Data are presented as mean values + /− SD. Three independent experiments were conducted for panels F-G with similar results. Source data are provided as a Source Data file.
