Fig. 1: SERINC genes are expressed in pneumocytes and their expression levels are unaffected by SARS-CoV-2 infection and type I IFN.
From: SARS-CoV-2 ORF7a potently inhibits the antiviral effect of the host factor SERINC5
a SERINC1, 2, 3, 4, and 5 RNA copy number relative to GAPDH in total lung RNA. Graphs represent mean ± SD from three independent donors. b SERINC1, 2, 3, 4, and 5 RNA copy number relative to GAPDH in Calu-3 cells. c Fold expression change of SERINC1, 2, 3, 5, and ISG15 transcripts relative to mock, normalized to GAPDH in Calu-3 cells treated with IFNβ (500 U/ml) for 4 h, 8 h, 16 h or 24 h. Mock indicates mock-treated (PBS). d SARS CoV-2 gRNA copies relative to GAPDH from Calu-3 cells infected with SARS-CoV-2 for 2 h or 6 h e SERINC1, 2, 3, and 5 transcripts fold expression from d relative to uninfected Calu-3 cells and normalized to GAPDH. All graphs represent mean ± SD from 3 independent experiments. (hours post infection, hpi; SARS-CoV-2, CoV-2).
