Fig. 3: SERINC5 restricts SARS-CoV-2 entry of different Spike variants.
From: SARS-CoV-2 ORF7a potently inhibits the antiviral effect of the host factor SERINC5
293T cells were co-transfected with HIV-1NLΔEnv-NanoLuc, SARS-CoV-2 Spike variants (Wuhan-Hu-1, alpha, beta, gamma, and delta) and either SERINC5 or empty vector (E.V.). Forty-eight hours post transfection, released virus in the culture media was used to infect Calu-3 cells. Luciferase levels were measured 48 hpi and normalized to HIV-1 p24CA levels of the input pseudovirus. Percent (%) of relative infectivity with respect to pseudovirus produced in the presence of E.V. is shown. All results are presented as mean ± SD from n = 3 independent experiments. Statistical significance was determined by one-sample t-test (two-tailed).
