Fig. 4: KDM5B leads to a differentiation-directed phenotypic shift.
From: Persister state-directed transitioning and vulnerability in melanoma
a, b Heatmaps of the SARRIO_EPITHELIAL_MESENCHYMAL_TRANSITION_DN36, GERBER_PROLIFERATION_SPOT A signatures37 (a) and GO_PIGMENTATION signature38,39 (b) from WM3734Tet3G-KDM5B cells after 72 h of Dox treatment and WM3734 and CSM152 cells after 72 h of Cpd1 treatment; red, upregulated; green downregulated genes. Significance is indicated by FWER p < 0.05. c Heatmap of the Tsoi differentiation trajectory66 for WM3734Tet3G-KDM5B cells after 24, 48, and 72 h Dox treatment (left), WM3734 KDM5B knockdown (sh) versus control (scr) cells (right); red, upregulated, green downregulated genes. d Violin plot displaying endogenous KDM5B expression levels according to the transcriptional differentiation level in single-cell RNA-sequenced human melanomas. Asterisks represent a p value <0.0001 from one-way ANOVA with Dunnett’s multiple comparison test. Here the comparison was made to the undifferentiated group.
