Fig. 4: Frequency of eructophagy is increased in IFN-γ-activated macrophages and further enhanced with mTOR inhibition. | Nature Communications

Fig. 4: Frequency of eructophagy is increased in IFN-γ-activated macrophages and further enhanced with mTOR inhibition.

From: Macrophages disseminate pathogen associated molecular patterns through the direct extracellular release of the soluble content of their phagolysosomes

Fig. 4: Frequency of eructophagy is increased in IFN-γ-activated macrophages and further enhanced with mTOR inhibition.The alternative text for this image may have been generated using AI.

a–c Percentages of phagolysosomes undergoing at least one eructophagic event over 5 h following phagocytosis of particles conjugated to DQ Green BSA, pHrodoSE, AF647SE. Rates of eructophagy are influenced by a prior exposure of BMMØs to IFN-γ (100 U/mL, 18 h) or IL-4 (10 ng/mL, 24 h). Rates of eructophagy in IFN-γ-treated BMMØs are influenced by the addition of b mTOR inhibitors (rapamycin or Torin 1) or c mTOR activators (1× MEM amino acids) 30 min post-phagocytosis. Error bars presented as means ± SEM. a, b *P ≤ 0.05 by one-way ANOVA with Bonferroni’s post hoc test. c P = 0.001 Student’s two-tailed unpaired t-test. a n = 5 independent experiments. b, c n = 3 independent experiments. Source data are provided as a Source Data file.

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