Fig. 5: Autophagic machinery is required for eructophagy. | Nature Communications

Fig. 5: Autophagic machinery is required for eructophagy.

From: Macrophages disseminate pathogen associated molecular patterns through the direct extracellular release of the soluble content of their phagolysosomes

Fig. 5

Eructophagy was detected by hydrolysis of the membrane impermeant substrate resorufin maltotriose (yellow) by α-amylase conjugated to 3.0 μm reporter particles within phagolysosomes between 1 and 3 h following phagocytosis by BMMØs. Particles are labelled with the reference fluor AF647SE (magenta). a Rates of eructophagy in BMMØs conditionally deficient in Beclin 1, ATG5, TSC1, ATG7 and ATG16L (Cre+) relative to BMMØs derived from Cre-negative littermates (Cre−). b Eructophagy is reduced in BMMØs deficient in Pi3Kγ, but not in Cybb or Rubicon relative to BMMØs derived from C57BL6/J mice (wild type; WT). c Representative images before, during and after an eructophagy event (yellow) in BMMØs derived from mice expressing LC3-GFP (green). LC3-GFP recruitment to the phagolysosome was observed to be temporally associated with 88% (±3.1%) of all eructophagy events observed in these cells. Error bars presented as means ± SEM. *P = (a) 0.0047, 0.0479, 0.0052, 0.0019 and 0.0004; (b) 0.0001, 0.5513 and 0.8169 by Student’s two-tailed unpaired t-test. Time 0 represents an eructophagy event. Scale bars denote 10 μm. a, b n = 3, n = 4 or n = 5 mice per group, as shown by the number of data points on the graph. Source data are provided as a Source Data file.

Back to article page