Fig. 1: Overview of workflow of globLoc algorithm.
From: Global fitting for high-accuracy multi-channel single-molecule localization

The overall workflow of globLoc can be divided into three parts: 1. calculation of multi-channel transformation using control points from beads or single molecule data; 2. generation of multi-channel PSF models by properly averaging beads from multiple channels; 3. fitting of the multi-channel single molecule data using global maximum likelihood estimation with flexible parameter linking.