Fig. 4: Performance of globLoc on ratiometric multi-color data.
From: Global fitting for high-accuracy multi-channel single-molecule localization
a Emission spectra of DY-634, AF647, CF660C and CF680 and transmission profile of the dichroic beamsplitter. The experimentally determined ratio of photons between dark and bright channels for these four dyes are 0.39, 0.21, 0.07 and 0.02. b Scatter plot of the fitted transmitted versus reflected photons per localization in a typical 4 color experiment using global fit and individual fit separately. c 4 color 3D imaging of Nup62-DY634, Nup96-AF647, ELYS-CF660C and WGA-CF680 in the NPC using globLoc. c1, Top view of bottom nuclear membrane. c2, Zoomed image of the region indicated in d1. c3, Top view and side view images of the region indicated in c2. c4, Average of 200 NPC images by registering the Nup96 structures that we used as a reference. See Supplementary Movie 1. Representative results are shown from 3 experiments for (c). Scale bars 10 µm (c1), 1 µm (c2), 100 nm (c3-4).
