Fig. 6: Inhibition of nuclear protein export interferes with mitochondrial quality control (MQC). | Nature Communications

Fig. 6: Inhibition of nuclear protein export interferes with mitochondrial quality control (MQC).

From: Mistargeting of aggregation prone mitochondrial proteins activates a nucleus-mediated posttranscriptional quality control pathway in trypanosomes

Fig. 6

a IF analysis of cells expressing ubiquitin-like protein 1 (TbUbL1-myc) with simultaneous atypical translocase of the outer membrane 69 (ATOM69)-RNAi, induced for 3d (left), and additionally treated with 50 ng/ml leptomycin B (LMB) for 1d (right). Scale bar 10 μm. The % of the population displaying a TbUbL1-myc nuclear release phenotype is recorded below the images, 100 cells of each were analysed. This IF analysis was repeated independently at least three times with similar results. b Immunoblots depicting the change in abundance of the substrates in situ tagged FtsH-HA and endogenous cytochrome oxidase subunit IV (COXIV) in whole cells after tetracycline-inducible RNAi mediated ablation of ATOM69 in the absence and presence of 50 ng/ml LMB. 2 × 106 cells were loaded per lane. Elongation factor 1a (EF1a) is used as a loading control. c Quantifications of the FtsH-HA and COXIV levels of triplicate immunoblots shown in b, with the tagged protein level of each uninduced cell line set to 100%. The level of tagged protein for each sample was normalised to its respective EF1a signal. Data are presented as mean values with error bars corresponding to the standard deviation of the mean of three independent biological replicates. Significance calculated using a two-tailed unpaired t-test, with p-values indicated. Source data are provided as a Source Data file.

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