Fig. 4: Parvalbumin blocker which blocks Parvalbumin and CSF1R interaction, promotes M2 macrophage polarization.
From: Targeting parvalbumin promotes M2 macrophage polarization and energy expenditure in mice
a Proposed docking interface between parvalbumin and CSF1R based on molecular docking. CSF1R and parvalbumin are shown in green and blue respectively. The fragment M141-Y154 of CSFR1 is highlighted in orange. The residues in hydrogen bond network are shown in sticks. b CSF1R E143A and R146A abrogated the interaction with parvalbumin. c Parvalbumin blocker (1 μg/ml) blocked parvalbumin-CSF1R interaction. d Effect of parvalbumin blocker on ELK1 luciferase reporter activity in 293 T transfected with CSF1R (concentrations of parvalbumin blocker: 0.1 μg/ml, 1 μg/ml, 10 μg/ml, n = 3 per group). Effect of M-CSF with or without parvalbumin was shown (n = 3 per group). P value: <0.0001, <0.0001 and 0.0009. Parvalbumin blocker rescued parvalbumin suppression of M2 macrophage activation. MCSF-induced mTORC2 downstream targets and ERK activation (concentrations of parvalbumin blocker: 0.1 μg/ml, 1 μg/ml, 10 μg/ml) (e) and M2 macrophage marker gene expression (concentrations of parvalbumin blocker: 1 μg/ml) (f) (n = 5 per group) in BMDM were shown. P value: 0.0003, 0.0100, 0.0415. Data are represented as mean ± SEM. P values were determined by unpaired two-tailed Student’s t test (d and f). *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.
