Fig. 1: Mouse-adaptation strategy and lung virus titers upon challenge with WT- and MA-SARS-CoV-2. | Nature Communications

Fig. 1: Mouse-adaptation strategy and lung virus titers upon challenge with WT- and MA-SARS-CoV-2.

From: Characterization of SARS-CoV-2 Spike mutations important for infection of mice and escape from human immune sera

Fig. 1

A The SARS-CoV-2 USA-WA1/2020 Seattle strain was obtained from BEI Resources and serially passaged 11 times in mice of different genetic backgrounds. B Sequence and location of mutations identified in MA-SARS-CoV-2 when compared to WT-SARS-CoV-2. C Infection with 2.5 × 104 PFU of the MA-SARS-CoV-2 resulted in detectable virus titers in lungs (right lung lobes were used for titration) and nasal turbinates at 3 DPI in female 129S1 mice whereas the WT-SARS-CoV-2 did not. D Infection with 2.5 × 104 PFU of MA-SARS-CoV-2 but not WT-SARS-CoV-2, resulted in detectable lung virus titers harvested at different time points post-infection in C57Bl6 as well as E BALB/c mice irrespective of sex. Bars represent geometric means. Dotted line indicates limit of detection (LOD) −66.67 PFUml−1. Each data point corresponds to one mouse and the number of data points represents the number of mice in the corresponding groups. Source data are provided as a Source Data file.

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