Fig. 4: Ectopic Lr42 expression and the EMS Lr42 mutant.
a Phenotype of Bobwhite was susceptible (N = 12, Infection Type = 3), phenotype of Ubip::Lr42 (Lr42 driven by the maize ubiquitin promoter) T2 transgenic plants (event 3) was hypersensitive fleck (N = 12, Infection Type = ;), and phenotype of two independent events of Lr42p::Lr42 (Lr42 driven by the native promoter of Lr42) were resistant upon inoculation with race TFBJG. Infection types of Lr42p::Lr42-1 (N = 3) and Lr42p::Lr42-2 (N = 9) were “;” and 2−, respectively. Transgenic plants of both Lr42p::Lr42 events were in the T1 generation, and Ubip::Lr42 plants were from the T2 generation. b qRT-PCR of the Lr42 expression in Bobwhite and transgenic plants. Lr42 expression of Bobwhite is undetectable. One plant for each biological replicate, and three biological replicates were used in gene expression analysis. Bar heights are means and error bars stand for standard deviations (SD). The quantification was repeated twice with consistent results. c Phenotype of M3 individuals from the TA2450 EMS mutant family that carried a G to A mutation at 2099 bp of the Lr42 coding region, causing the C700Y amino acid substitution. The genotype (GG, GA, or AA) at the mutation site of each plant individual was listed above the leaf. Seedling plants were inoculated with race PNMRJ. Source data are provided as a Source Data file.
