Fig. 6: Introgression of the Lr42 segment in CIMMYT wheat lines.

a Each point represents a wheat line with the log10 of total count of GBS tags per line on the x-axis and the number of Lr42-specific GBS tags on the y-axis. Green and blue colors signify Lr42+ and Lr42− Lr42-introgressed lines, respectively. Other lines that are either not Lr42-introgressed lines or not confidently categorized into Lr42+ or Lr42− are gray colored. Numbers of Lr42-specific GBS tags were jittered with the factor 0.25 for better visualization of data density. b, c Using disease phenotypic data collected at CIMMYT, statistical comparisons of disease infection types between Lr42+ and Lr42− lines were performed for seedling stage leaf rust with two-side t-test and for adult stage resistance with ANOVA. Two field locations in Mexico for adult stage leaf rust phenotyping are labeled. The Stakman 0–4 scale was linearized to a 0–9 scale for seedlings (Methods). The percent disease severity on adult plant flag leaves was rated on the Cobb scale. Bars represent standard deviation of means of infection types or disease severity. Gray open dots represent single data points.