Fig. 3: Tyrosine and cis-RSV modulate the de novo synthesis of TyrRS at the elongation step of protein synthesis. | Nature Communications

Fig. 3: Tyrosine and cis-RSV modulate the de novo synthesis of TyrRS at the elongation step of protein synthesis.

From: Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration

Fig. 3

a Low and high concentrations of trans-RSV (10 and 50 μM) have opposite effects on TyrRS, and low concentration trans-RSV mimics cis-RSV. Representative immunoblots for TyrRS after treatment with cis-and trans-RSV (10 and 50 μM) in rat cortical neurons (DIV 9). b cis-and trans-RSV have opposite effects on TyrRS. Representative spectral images (scale bar, 20 µm) of TyrRS in rat cortical neurons (DIV 10) following treatment with either cis-RSV or trans-RSV for 16 hr (MAP2 – neurite, magenta; DAPI – nucleus, blue; TyrRS – red-yellow spectral image). c cis-RSV protects neurons against trans-RSV-mediated decrease in TyrRS. Representative immunoblots showing TyrRS after treatment with trans-RSV (50 μM) alone or combined with different doses of cis-RSV for 4 hr. d cis-RSV protects neurons against tyrosine-mediated depletion of TyrRS. Cortical neurons were treated with Tyr (200 μM) alone or combined with cis-RSV for 8 hr. e cis- and trans-RSV have opposite effects on neuronal PheRSβ. Representative immunoblots for PheRSα/β after treatment with cis-and trans-RSV (5–50 μM) for 4 hr. f cis-RSV stimulates the de novo synthesis of TyrRS. Spectral images (scale bar, 20 µm) after treatment with cis-RSV (50 μM) for 1 hr either alone or combined with CHX (100 μg). g cis- and trans-RSV have opposite effects on the phosphorylation of eEF2. Representative immunoblots for p-eEF2 after treating with cis- and trans-RSV (5–50 μM) for 8 hr. h trans-RSV inhibits global protein synthesis. Cortical neurons were treated with cis-and trans-RSV (5–50 μM) for 1 hr, followed by puromycin incorporation and detection using anti-puromycin antibody. i cis-RSV facilitates the interaction of PP2A with eEF2. Cortical neurons were treated with cis- and trans-RSV (50 μM) or Tyr (200 μM) either alone or combined with cis-RSV for 4 hr. Immunoprecipitated eEF2 was probed for its interaction with PP2A and TyrRS. All data represent mean ± SEM for n = 3 experiments with significance measured using two-way ANOVA with Tukey’s test for multiple comparisons. Source data are provided as a Source Data file.

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