Fig. 8: Single-cell RNA sequencing reveals that rhIL-7-hyFc preferentially expands IL-7Rα effector memory CAR T cells.
a RamosCBR/GFP bearing NSG mice (5 × 105 cells IV on day −4) received rhIL-7-hyFc on days +1 and +15 following 1 × 106 UCART19 injection. Spleens were harvested on days +14 and +28, and UCART19 cells enriched using CD34+ magnetic selection were subject to single-cell RNA-sequencing. b Proportion of cells (size of circle) for each single-cell cluster (Seurat) were broken down by treatment group (Input, Week 2, and Week 4). c UMAP representation of the single-cell RNA-sequencing data, combined from all samples, colored and labeled by Seurat clusters. d Downsampled objects (n = 2000 cells for each treatment group) separated by treatment week. e Downsampled objects (n = 2000 cells for each treatment group) colored by exhaustion score, calculated based on the expression of genes known to be involved in T cell exhaustion (CTLA4, PDCD1, LAG3, HAVCR2, CD160, CD244, and TIGIT). Dark purple indicates a higher exhaustion phenotype, and light blue indicates a less exhaustive phenotype. f Gene expression analysis of relevant T cell genes and pathways for each Seurat cluster. The size of the circle indicates the percentage of cells with the expression of each gene and color indicates average expression across the cells in that unique cluster. g Focused view of clusters 0 and 1 showing increased expression of the genes IL-7Rα, GZMB, S100A4, and PTPRC across treatment groups.
