Fig. 3: Hormone exposure increases metastatic spread. | Nature Communications

Fig. 3: Hormone exposure increases metastatic spread.

From: Estrogen receptor positive breast cancers have patient specific hormone sensitivities and rely on progesterone receptor

Fig. 3

a–c Relative ex vivo radiance in different organs of mice engrafted with a MCF7 median n = 15. b T47D median n = 6. c HCC1428 median n = 2 after 45-day-hormone treatment. Bar graphs indicate average ± SEM; two-way ANOVA with Dunnett’s multiple comparison test. d–f Bar graphs showing relative metastatic burden in different organs of MCF7, T47D, and HCC1428 xenograft-bearing mice treated with E2, P4, or E2 + P4 average ± SEM. Kruskal–Wallis test, followed by Dunn’s multiple comparison test. d CTRL n = 59, E2 n = 60, P4 n = 44, E2 + P4 n = 56. e CTRL n = 24, E2 n = 24, P4 n = 24, E2 + P4 n = 24. f CTRL n = 8, E2 n = 8, P4 n = 8, E2 + P4 n = 12. g Heatmap showing relative metastatic load in mice xenografted with different patients’ tumors. Data represent mean fold change radiance compared to CTRL for each PDX. Friedman test, followed by Dunn’s multiple comparison test. T99, CTRL n = 3, E2 n = 6, P4 n = 4, E2 + P4 = 6. T105, CTRL n = 5, E2 n = 4, P4 n = 4, E2 + P4 = 5. T109, CTRL n = 3, E2 n = 6, P4 n = 4, E2 + P4 = 4. T110, CTRL n = 4, E2 n = 2, P4 n = 4, E2 + P4 = 3. T111, CTRL n = 3, E2 n = 3, P4 n = 3, E2 + P4 = 2. T113, CTRL n = 3, E2 n = 2, P4 n = 2, E2 + P4 = 1. PL-011, CTRL n = 3, E2 n = 2, P4 n = 3, E2 + P4 = 2. PL-015, CTRL n = 4, E2 n = 4, P4 n = 4, E2 + P4 = 3. h Bar graph of relative metastatic burden per organ in mice engrafted via teats with different PDXs after 60-day-hormone treatment. Data represent mean ± SEM. CTRL n = 24, E2 n = 21, P4 n = 22, E2 + P4 n = 21, mixed effects analysis, and post hoc multiple comparisons. i Bar plot of relative metastatic burden in PDXs bearing mice under hormone treatment. Data represent mean ± SEM. CTRL n = 96, E2, P4, E2 + P4 n = 84. Holm–Sidak’s multiple comparisons test. j Bar graph of relative growth at endpoint in mice engrafted with PDXs. Data represent mean ± SEM. CTRL n = 24, E2 n = 21, P4 n = 22, E2 + P4 n = 21, mixed effects analysis, and post hoc multiple comparisons.

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