Fig. 1: Severe immunopathology with necrotic granulomas in Mtb K and LCMV Arm-coinfected mice and differential pathogenesis of coinfected mice depending on the Mtb strain. | Nature Communications

Fig. 1: Severe immunopathology with necrotic granulomas in Mtb K and LCMV Arm-coinfected mice and differential pathogenesis of coinfected mice depending on the Mtb strain.

From: Viral coinfection promotes tuberculosis immunopathogenesis by type I IFN signaling-dependent impediment of Th1 cell pulmonary influx

Fig. 1

a C57BL/6 mice were infected with Mtb alone or with Mtb followed by subsequent infection with LCMV Arm at 14 days post Mtb infection. At 31 days post Mtb infection, the mice were sacrificed. b Gross pathology or c, H&E staining of lungs in each group. d Acid-fast staining of the lung in each group. (10x, scale bars, 2 mm; 400x, scale bars, 50 μm). e The weight of mice at the indicated time points (n = 4 or 5). f The data represent the percentage of the superior lobe of the right lung showing inflammation and are shown as center, bounds of box-and-whisker and percentile plots showing the minimum and maximum values (n = 5). g, Bacterial load in the organs of each group (n = 5). C57BL/6 mice were infected with the indicated Mtb strains. Some mice from each group were subsequently infected 2 weeks later with LCMV Arm. At 4.5 weeks post Mtb infection, the mice were sacrificed. h Gross pathology or i H&E staining of lungs in each group. Scale bars, 2 mm. j Bacterial load in the lungs of each group. Data were analyzed by e–g, two-tailed unpaired Student’s t test. Graphs show the mean ± SEM. b, c, d, f, g, The data are representative of at least two independent experiments or h–j, a single experiment. Source data are provided as a Source Data file.

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