Fig. 5: Rescued CXCL9 expression through type I IFN receptor blockade in pulmonary regions. | Nature Communications

Fig. 5: Rescued CXCL9 expression through type I IFN receptor blockade in pulmonary regions.

From: Viral coinfection promotes tuberculosis immunopathogenesis by type I IFN signaling-dependent impediment of Th1 cell pulmonary influx

Fig. 5

Seven days post LCMV infection, we analyzed CXCL9 expression in the lung by multiplex immunofluorescence staining. a Representative images of CXCL9 (yellow) immunofluorescence and DAPI (blue) staining in noninflammatory or inflammatory regions of lung tissues. Scale bars, 800 μm or 50 μm. b Representative images of CXCL9 and DAPI staining in lung tissues. Scale bars, 30 μm. c The number of CXCL9+ cells in each randomly selected region was counted and is summarized in the bar graphs (n = 4). One-way ANOVA with post hoc Tukey’s test. Plots show the mean ± SEM. d Representative images of CXCL9 (yellow), CD11b (white), CD31 (magenta), and PDPN (green) immunofluorescence and DAPI (blue) staining of lung tissues. Scale bars, 40 μm. The data are representative of single experiments. Source data are provided as a Source Data file.

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