Fig. 7: Curtailed migration of Mtb-specific T cells by negative regulation of chemokines by type I IFN. | Nature Communications

Fig. 7: Curtailed migration of Mtb-specific T cells by negative regulation of chemokines by type I IFN.

From: Viral coinfection promotes tuberculosis immunopathogenesis by type I IFN signaling-dependent impediment of Th1 cell pulmonary influx

Fig. 7

a The frequency and b, number of Ly5.1+ donor P25 cells in the dLNs and lungs were analyzed by flow cytometry and are summarized in the graph. a (n = 4, 5, 10, 11, 12, 13) or b, (n = 8, 9, 14, 15). At day 7 post LCMV Arm infection, c CXCL9 or d CXCL10 protein and RNA levels in lung homogenates were analyzed by ELISA and qRT-PCR, respectively (n = 5, 11, 15). The correlations between chemokine levels and e, the frequency of P25 cells in the lung (n = 4, 11, 13), f, IFN-γ+ cells in the lung (n = 4, 11, 13) and g, bacterial loads were analyzed (n = 5, 11, 14). h–j The CXCL9 or CXCL10 protein and RNA levels in the culture supernatant of RAW264.7 cells in the indicated groups treated with type I IFNs and type II IFN. The data were analyzed by a–d, h, one-way ANOVA with post hoc Tukey’s test or e–g, two-way test with Pearson’s correlation. Plots show the mean ± SEM. a–g, The data are pooled from two independent experiments or h, representative of at least two independent experiments (n = 3, 5 wells/group) or i, j, representative of a single experiment. Source data are provided as a Source Data file.

Back to article page