Fig. 4: Investigation on activation mechanism using scavengers.
a Quenchers and converters used to highlight the impact of specific radicals on cis-GdAzo-activation efficacy upon XR, GR and E (cf. Supplementary Section 7 for details). b Absorbance difference (365 nm) of medium containing cis-GdAzo (50 µM) in the presence of tBuOH (10, 100, 524 mM (∼5% v/v), control in water) before and after GR irradiation (n = 2 independent experiments). c Molecular activation of cis-GdAzo (50 µM, H2O) upon GR irradiation in N2 or N2O-saturated solutions (Control without inerting) determined by HPLC and reported as the difference of trans-isomer proportion before and after GR irradiation (n = 3 independent experiments). d Comparison of cis-GdAzo activations in control (no inerting) and N2O-saturated solutions in conjunction with the equivalent (eq.) amount of hydroxyl radicals HO• generated depending on doses (“eq 4”, “eq 10”, “eq 20” and “eq 40” relate to the doses of 2, 5, 10, and 20 Gy in N2O saturated solutions) (n = 3 independent experiments). The means ± standard deviations are reported. OD optical density.
