Fig. 7: During the re-warming process, adipocyte HIF2α deficiency fails to mitigate the mitochondrial activity.
From: Adipocyte HIF2α functions as a thermostat via PKA Cα regulation in beige adipocytes
a Experimental scheme (top) and western blot analysis of HIFα and UCP1 in iWAT of upon cold exposure or re-warming (bottom). b Experimental scheme of re-warming. c Representative images of H&E staining of iWAT from WT and HIF2α AKO mice upon cold exposure or re-warming (cold 2 weeks + TN 1 week). Scale bars, 100 μm. d miR-3085-3p level of iWAT from WT (n = 6) and HIF2α AKO (n = 5) mice upon cold and re-warming (cold 2 week + TN 1 week). e, f mRNA levels in iWAT from WT (n = 6) and HIF2α AKO (n = 5) mice upon cold exposure and re-warming (cold 2 weeks + TN 1 week). g Western blot analysis of OXPHOS complexes, PKA Cα, and UCP1 in iWAT from WT and HIF2α AKO mice upon cold exposure and re-warming (cold 2 weeks + TN 1 week). h Experimental scheme of re-warming with con or miR-3085-3p mimic injection. i Representative images of H&E staining of iWAT from WT and HIF2α AKO mice upon re-warming (cold 2 weeks + TN 1 week) with con or miR-3085-3p mimic injection. Scale bars, 50 μm. j Western blot analysis of OXPHOS complexes, PKA Cα, and UCP1 in iWAT from WT and HIF2α AKO mice upon re-warming (cold 2 weeks + TN 1 week) with con or miR-3085-3p mimic injection. Data were expressed as the mean ± SEM by two-tailed unpaired Student t-tests in (d) or two-way ANOVA in (e, f) followed by Holm–Sidak’s multiple comparisons test.
