Fig. 1: Identification of nucleosome positioning and spacing with MNase-seq.
From: Dynamic nucleosome landscape elicits a noncanonical GATA2 pioneer model
a A scheme for generating multi-omics sequencing data including MNase-seq, MNase-ChIP-seq, and ChIP-exo for detecting nucleosome footprints. b A Pearson correlation of raw read counts within a bin size of 200 bp between two MNase-seq biological replicates in LNCaP cells showing a high coefficient value at 0.95 and two-tailed p value of 2.18e-16, illustrated by a representative chromosome 10. c Nucleosome density distribution between two replicates within a range of 5 Kb in each of 23 chromosomes. d Accumulation of nucleosome dyad according to the Mono, Di-, Tri-, and Penta-nucleosomes. Each nucleosome dyad was set as 0 bp and MNasse-seq reads in 600 bp upstream and downstream of each nucleosome dyad were used for plotting the accumulation. e The frequency distribution of the distance between adjacent nucleosomes dyad under 400 bp and an overall nucleosome spacing with a peak of 187 bp.
