Fig. 2: Dietary restriction delays visual senescence in a CLK-dependent manner.

a GO enrichment scores corresponding to downregulated light-response genes in heads from RNA-Seq of nCLK-Δ1 (Elav-GS-GAL4 > UAS-CLK-Δ1) vs controls. P-values were calculated with hypergeometric distribution (findGO.pl, HOMER) with no adjustment for multiple-hypothesis testing. b Heatmap of normalized RNA-Seq expression corresponding to the gene-ontology category “Deactivation of rhodopsin-mediated signaling” (GO:0016059) in nCLK-Δ1 and controls at zeitgeber times 0 and 12 (lights on and lights off, respectively). c Positive phototaxis responses for nCLK-Δ1 flies. For each timepoint results are represented as mean values of percent positive phototaxis ±SEM (n = 24 biologically independent cohorts of 20 flies examined over three independent experiments, N = 480 flies per condition). P-values were determined by two-tailed Student’s t-test (unpaired), comparing responses between diet and/or genotype at each timepoint. d Boxplots of electroretinogram amplitudes for nCLK-Δ1 flies and controls at day 14 and 21. Data are presented as Tukey multiple comparison of means: The horizontal line within each box is the median, the bottom and top of the box are lower and upper quartiles, and the whiskers are minimum and maximum values. (n = 8 and 12 biologically independent flies measured at day 14 and 21, respectively, examined over 1 independent experiment). P-values were determined by two-tailed Student’s t-test (unpaired), comparing responses between diet and/or genotype at each timepoint. Source data are provided as a Source Data file.