Fig. 3: Photoreceptor clocks modulate visual function and degeneration with age.

a Positive phototaxis responses for prCLK-Δ1 flies (Trpl-GAL4; GAL80^ts > UAS-CLK-Δ1), prCLK-OE (Trpl-GAL4; GAL80^ts > UAS-Clk), and control flies (Trpl-GAL4; GAL80^ts /+ ) reared at 30 °C. For each timepoint results are represented as mean values of percent positive phototaxis ±SEM (control and prCLK-Δ1: n = 24 biologically independent cohorts of 20 flies examined over three independent experiments, N = 480 flies per condition. prCLK-OE: n = 16 biologically independent cohorts of 20 flies examined over 2 independent experiments, N = 320 flies per condition). P-values were determined by two-tailed Student’s t-test (unpaired), comparing responses between diet and/or genotype at each timepoint. b Boxplots of electroretinogram amplitudes for prCLK-Δ1, prCLK-OE, and control flies reared at 30 °C. The sample sizes (n) corresponding to (b) can be found in the “Statistics and Reproducibility” section within the Methods. Data are presented as Tukey multiple comparison of means: The horizontal line within each box is the median, the bottom and top of the box are lower and upper quartiles, and the whiskers are minimum and maximum values. P-values were determined by two-tailed Student’s t-test (unpaired), comparing responses between diet and/or genotype at each timepoint. c Tangential sections through prCLK-Δ1, prCLK-OE, and control retinas at day 2 and day 10. R1-7 photoreceptors are apparent within each hexongonally shaped ommatidia. White bars in bottom right corners represents 10 microns. The number of biologically independent replicates for each group within this experiment are in the Statistics and Reproducibility section. Additional representative images are included with the source data file. Source data are provided as a Source Data file.