Fig. 5: Photoreceptor activation modulates lifespan in a diet-dependent fashion.

a Survival analysis of w¹¹¹⁸ flies housed in 12:12 h LD or constant darkness (DD). Survival data are plotted as an average of three independent lifespan repeats. b–f Survival analysis of w¹¹¹⁸; ninaE¹⁷, w¹¹¹⁸; rh3², w¹¹¹⁸; rh4¹, w¹¹¹⁸; rh6^G, and w¹¹¹⁸; Gqα¹ mutants compared to w¹¹¹⁸ control flies housed in 12:12 h LD. Survival data are plotted as an average of three independent lifespan repeats. *Survival curves for w¹¹¹⁸ are re-plotted (b–f) for visual comparison, and the w¹¹¹⁸ and rhodopsin-null lifespans repeats were performed simultaneously. All mutant lines were outcrossed to w¹¹¹⁸. g Hazard ratios for rhodopsin and Gq mutant flies compared to w¹¹¹⁸ control flies (ratios < 1 indicate flies that are more likely to survive compared to w¹¹¹⁸). The hazard ratio for each strain is plotted as the measure of centre and the error bars indicate the 95% confidence interval of the hazard ratios. P-values were determined by Log-rank (Mantel-Cox) test, ns denotes a non-significant P-value, **** indicates P ≤ 1.0e-15. h Survival analysis of eye-specific arr1-RNAi knockdown flies vs RNAi control flies. Survival data are plotted as an average of two independent lifespan repeats for arr1-RNAi and one independent lifespan replicate for RNAi-controls. i Survival analysis of retinal inducible, photoreceptor-specific optogenetic flies (Trpl-GAL4 > UAS-csChrimson [red-shifted]) supplemented with retinal or vehicle control and housed in 12:12 h red light:dark. Survival data are plotted as an average of two independent lifespan repeats. j Survival analysis of eye-specific ATPα RNAi knockdown flies vs RNAi control flies. Survival data are plotted as an average of three independent lifespan repeats. The total number of flies (N) corresponding to each lifespan in Fig. 5 can be found in the “Statistics and Reproducibility” section within the Methods. Source data are provided as a Source Data file.