Fig. 7: Spatiotemporal regulation of silk protein synthesis.
From: A single-cell transcriptomic atlas characterizes the silk-producing organ in the silkworm
a Chord diagram of the distribution of major silk protein-coding genes in each cell type. b Immunofluorescence staining for silk protein-coding genes in 1L1D and 1LM. The red part of the silk gland shows the location of the protein distribution. The white arrows demarcate the three regions of the silk gland. Nuclei were stained with DAPI (n ≥ 10 in three independent experiments). 1L1D, day 1 of the first instar; 1LM, first larval molting. c Representative GO terms enriched in sericin protein-synthesizing cells (SPSs), endoplasmic reticulum stress signaling cells (ERSSs), and sericin protein catabolism cells (SPCs) that regulate sericin protein synthesis. P values are shown in the charts are determined by multiple hypothesis testing. Source data are provided in Supplementary Data 14. d–f Top 20 KEGG pathways enriched in fibroin protein-synthesizing cells (FPSs), death and remodeling regulation cells (DRRs), and fibroin protein catabolism cells (FPCs) that regulate fibroin protein synthesis (p < 0.05). P-value was calculated by the hypergeometric distribution. Color gradients indicate the range of p-values. The p-values of each pathway are provided in Supplementary Data 15.
