Fig. 2: SAM boost immunization drives increased antigen-specific T cells and neutralizing antibody titers. | Nature Communications

Fig. 2: SAM boost immunization drives increased antigen-specific T cells and neutralizing antibody titers.

From: Low-dose self-amplifying mRNA COVID-19 vaccine drives strong protective immunity in non-human primates against SARS-CoV-2 infection

Fig. 2

Mice received SAM Spike(V2)-F2P prime immunization, followed by boost 8 weeks post prime (10 μg each). N = 4 mice/group. A T-cell response at the specified timepoint, assessed by IFNγ ELISpot following overnight stimulation with eight peptide pools spanning spike antigen. Mean ± SEM for each peptide pool for N = 4 independent animals per group; representative data from one of four similar studies, see additional data in Supplementary Fig. 3. Annotated numbers are the average sum of all eight peptide pools for each animal. Unpaired Student’s t-test (two-tailed) comparing sum of pools for each animal at each timepoint. Immunizations for each group were staggered to enable splenocyte collection and measurement of multiple timepoints post immunization in the same assay. B Pseudovirus neutralizing titer (NT50) at the specified timepoint. Geometric mean and geometric SD of N = 4 mice per group for one experiment. Representative data from one of three similar studies. Sera from Naive mice were below LOD of 25. Two-tailed Mann–Whitney test.

Back to article page