Fig. 3: Binding mode of CE in WT AtAHAS and in the W574L mutant.
From: Structural basis of resistance to herbicides that target acetohydroxyacid synthase
a Stereoview of the CE binding site in W574L. ThDP has been oxidized to thiamine thiazolone diphosphate (ThThDP, light brown). Electron densities (2Fo-Fc map) in light brown correspond to CE, ThThDP and L574 contoured at 1, 2, and 1σ, respectively. b Interactions between CE and WT AtAHAS (PDB code: 1YBH38). In this structure, ThDP is almost completely degraded and therefore not observed in the density map. c Interactions between CE and the W574L mutant. (All panels) CE is green and in ball and stick representation. FAD is yellow. Dashed lines represent hydrogen bonds (black). The grey dashed cylinders represent π–π and carbon–π interactions. The lime dashed cylinders represent halogen–π interactions. The cyan dashed cylinders represent hydrophobic interactions. Q-loop is a loop in the active site that includes residues 193 to 210. ThThDP thiamine thiazolone diphosphate.
