Fig. 4: Conformational changes to CE that are induced by the W574L mutation.
From: Structural basis of resistance to herbicides that target acetohydroxyacid synthase
a Stereo view of the herbicide binding site in the W574L:CE complex. The AHAS polypeptide is in light blue. FAD, ThThDP, and CE are in yellow, light brown, and green carbon atoms, respectively. The black thin lines represent CE and W574 in the AtAHAS-CE structure that has been superposed to the W574L-CE structure. The black arrow indicates the rotation of the pyrimidine ring of CE. The 2Fo-Fc electron density map is in gray mesh. The position of CE relative to (b) ThDP-peracetate and (c) ThAthDP and peracetate as they appear in the ScAHAS-PS (PDB code: 5WKC11) complex superimposed with the protein and herbicide coordinates of the W574L-CE structure. Thin dashed lines represent hydrophobic interactions. ThAthDP thiamineaminoethenethiol diphosphate.
