Fig. 2: Experimental setup of the tubulin pulling assay using an optical trap. | Nature Communications

Fig. 2: Experimental setup of the tubulin pulling assay using an optical trap.

From: The force required to remove tubulin from the microtubule lattice by pulling on its α-tubulin C-terminal tail

Fig. 2: Experimental setup of the tubulin pulling assay using an optical trap.The alternative text for this image may have been generated using AI.

Tubulin labeled with an oligo-DNA handle using the SSL method was linked to a long DNA linker through hybridization. Tubulin conjugated to the DNA-linker was polymerized with biotinylated tubulin and affixed to the surface of neutravidin-coated coverslips. The anti-digoxigenin antibody-coated microspheres were then bound to the DNA-linker labeled with digoxigenin. The mechanical force was exerted onto the α-tubulin CTT by pulling the microsphere with the optical trap along the y-axis, which is perpendicular to the long axis of microtubule (x-axis).

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