Table 1 Summary of GS phosphorylation site analysis.
From: Mechanism of glycogen synthase inactivation and interaction with glycogenin
Site | Sequence | Peptide modifications | ptmRS score | MASCOT score | Enzyme | PhosphoSite: | |
|---|---|---|---|---|---|---|---|
LTP | HTP | ||||||
S8 | PLNRTLsMS | S7(P) | S7: 100 | 40 | Elastase | 15 | 1 |
S8 | PLNRTLsMS | S7(P); M8(O) | S7: 100 | 31 | Elastase | 15 | 1 |
S8 | sMSSLPGLEDW | S1(P) | S1: 99.71 | 13 | Chymotrypsin | 15 | 1 |
S412 | ESLLVGsLPDMNKMLDKEDF | S7(P) | S7: 100 | 31 | Chymotrypsin | — | 17 |
S412 | ESLLVGsLPDMNKML | S7(P); M11(O); M14(O) | S7: 100 | 23 | Chymotrypsin | — | 17 |
S641 | QGYRYPRPAsVPPSPS | S10(P) | S10: 99.99 | 20 | Elastase | 32 | 30 |
S641 & S645 | QGYRYPRPAsVPPsPS | S10(P); S14(P) | S10: 100; S14: 100 | 24 | Elastase | (645) 21 | (645) 39 |
S652 | RHsSPHQSEDEEDPRNGPL | S3(P) | S3: 99.78 | 34 | Elastase | — | 15 |
S652 & S653 & S657 | RHssPHQsEDEEDPRNGPL | S3(P); S4(P); S8(P) | S3: 100; S4: 100; S8: 100 | 19 | Elastase | (653) 13 | (653) 27 |
S652 & S657 | RHsSPHQsEDEEDPRNGPL | S3(P); S8(P) | S3: 99.67; S8: 100 | 53 | Elastase | (657) 14 | (657) 50 |
S727 | RNSVDTATSSSLSTPSEPLsPTSSLGEER | S20(P) | S20: 100 | 66 | Trypsin | 1 | 22 |
S727 | STPSEPLsPTSSL | S8(P) | S8: 99.63 | 20 | Chymotrypsin | 1 | 22 |
S731 | STPSEPLSPTSsLGEERN | S12(P) | S12: 99.79 | 73 | Chymotrypsin | — | 6 |
S731 | TPSEPLSPTSsL | S11(P) | S11: 100 | 22 | Elastase | — | 6 |
S727 & S731 | NSVDTATSSSLSTPSEPLsPTSsLGEER | S19(P); S23(P) | S19: 100; S23: 99.52 | 60 | Trypsin | See above | |
S727 & S731 | STPSEPLsPTSsLGEERN | S8(P); S12(P) | S8: 100; S12: 100 | 42 | Chymotrypsin | See above | |
